CMHD Logo Toronto Centre for Phenogenomics  
Centre for Modeling Human Disease
Home Contact Us Careers at TCP Sitemap TCP Calendar



Adissu H, Fang H, McKerlie C, Khokha R, Wood G. Tissue inhibitor of metalloprotease-3 (TIMP3) deficiency promotes invasive prostatic carcinoma. 1st Cancer Research Symposium, The Institute for Comparative Cancer Investigation, University of Guelph, Guelph, Canada. June 2010.

Adissu HA, McKerlie C, Khokha R, Wood GA. Tissue inhibitor of metallopreotease-3 (TIMP3) deficiency promotes invasive prostate adenocarcinoma. The Hospital for Sick Children Physiology & Experimental Medicine Research Program Scientific Retreat, Toronto, Canada. March 2010.

Wang Y, Newbigging S, Morikawa L, McKerlie C. Fixation and perfusion of the mouse. The Hospital for Sick Children Physiology & Experimental Medicine Research Program Scientific Retreat, Toronto, Canada. March 2010.


Schofield PN, et al., McKerlie C, et al. (CASIMIR Meeting Rome Participants). Opinion - Post-publication sharing of data and tools. Nature 461 (7260): 171-173. 10 September 2009.

Ng D, Pitcher GM, Szilard RK, Sertié A, Kanisek M, Clapcote SJ, Lipina T, Kalia LV, Joo D, McKerlie C, Cortez M, Roder JC, Salter MW, McInnes RR. Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning. PLoS Biol 7 (2): e1000041. February 2009.

Schofield PN, Brown SDM, Sundberg JP, Warren M, Dubus P, Ellender M, Fiette L, Rozell B, Quintanilla-Martinez L, Raspa M, Song J-Y, van der Valk M, Arends M, McKerlie C. Letter to the Editor - PRIME importance of pathology expertise. Nat Biotechnol 27 (1): 24-25. January 2009.


Deng H, Tang N, Stief AE, Mehta N, Baig E, Head R, Sleep G, Yang XZ, McKerlie C, Trudel S, Stewart AK, McCart JA. Oncolytic virotherapy for multiple myeloma using a tumour-specific double-deleted vaccinia virus. Leukemia 22 (12): 2261-2264. December 2008.

Yang Y, McKerlie C, Wang LC, Lu Zhan, Buchwald M. In vivo potential effects of adenovirus type 5 E1A and E1B on lung carcinogenesis and lymphoproliferative inflammation. J Virol 82 (16): 8105-8111. August 2008.

Grasso F, Engelberts D, Helm E, Frndova H, Jarvis S, McKerlie C, Babyn P, Post M, Kavanagh BP. Negative-pressure ventilation: Better oxygenation and less lung injury. Am J Respir Crit Care Med 177(4): 412-418. February 2008.

Nemati B, Atmodjo W, Gagnon S, Humes D, McKerlie C, Kaplan F, Sweezey NB. Glucocorticoid receptor disruption delays structural maturation in the lungs of newborn mice. Pediatr Pulmon 43(2): 125-133. February 2008.


Lee SU, Grigorian A, Pawling J, Chen IJ, Gao G, Mozaffar T, Mckerlie C, Demetriou M. N-glycan processing deficiency promotes spontaneous inflammatory demyelination and neurodegeneration. J Biol Chem 282(46): 33725-34. November 2007.

Chen N-J, Mirtsos C, Suh D, Lu Y-C, Lin W-J, McKerlie C, Lee T, Baribault H, Tian H, Yeh W-C. C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a. Nature 446 (71322): 203-207. March 2007.

Roth-Kleiner M, Ridsdale R, Cao L, Kuliszewski M, Tseu I, McKerlie C, Post M. Lipopolysaccharide exposure modifies high tidal volume ventilation-induced proinflammatory mediator expression in newborn rat lungs. Ped Res 61(2): 191-196. February 2007.

Tremblay LO, Kovács EN, Daniels E, Wong NK, Sutton-Smith M, Morris HR, Dell A, Marcinkiewicz E, Seidah NG, McKerlie C, Herscovics A. Respiratory distress and neonatal lethality in mice lacking Golgi ?1,2-mannosidase IB involved in N-glycan maturation. J Biol Chem 282 (4): 2558-2566. January 2007.


Meyer J, Cox PN, McKerlie C, Bienzle D. High frequency oscillatory ventilation in a rabbit model. Ped Res 60 (4): 401-406. October 2006.

Tsuchida S, Engelberts D, Peltekova V, Hopkins N, Frndova H, Babyn P, McKerlie C, Post M, McLoughlin P, Kavanagh BP. Atelectasis causes alveolar injury in non-atelectatic lung regions. Am J Respir Crit Care Med 174 (3): 279-289. August 2006.

Caron J, Lariviére L, Nacache M, Tam M, Stevenson MM, McKerlie C, Gros P, Malo D. Influence of Slc11a1 on the outcome of Salmonella enterica serovar enteritidis infection in mice is associated with Th polarization. Infect Immun 74 (5): 2787-2802. May 2006.

Gagnon S, Atmodjo W, Humes D, McKerlie C, Kaplan F, Sweezey NB. Transgenic glucocorticoid receptor expression driven by the SP-C promoter reduces neonatal lung cellularity and midkine expression in GRhypo mice. Biol Neonate 90 (1): 46-57. March 2006.

McKerlie C. Cause and effect considerations in pathology diagnosis and pathology phenotyping of genetically engineered mice. ILAR J (Nat Acad Sci) 47 (2): 155-161. March 2006.

Wang Y, Morikawa L, McKerlie C. Laser capture microdissection: Getting it to work. 32nd Annual Meeting of the National Society of Histotechnology, San Antonio, USA. September 2006.

McKerlie C. Toronto Centre for Phenogenomics. 45th Annual Meeting Canadian Society for Laboratory Animal Science (CALAS), Montreal, Canada. April 2006.


Wang Y, Vachon E, Zhang J, Cherepanov V, Kruger J, Li J, Saito K, Shannon P, Bottini N, Huynh H, Ni H, Yang H, McKerlie C, Quaggin S, Zhao ZJ, Marsden PA, Mustelin T, Siminovitch K, Downey G. Tyrosine phosphatase MEG2 modulates murine development and platelet and lymphocyte activation through secretory vesicle function. J Exp Med 202 (11): 1587-1597. December 2005.

Koehler DR, Frndova H, Leung K, Louca E, Palmer D, Ng P, McKerlie C, Cox P, Coates A, Hu J. Aerosol delivery of an enhanced adenoviral formulation to rabbit lung with an intratracheal catheter. J Gene Med 7(11):1409-20. November 2005.

Flenniken AM, Osborne LR, Anderson N, Ciliberti N, Fleming C, Gittens JEI, Gong X-Q, Lounsbury C, Moreno L, Nieman BJ, Peterson K, Qu D, Roscoe W, Shao Q, Tong D, Veitch GIL, Voronina I,Vukobradovic I, Wood G, Zhu Y, Zirngibl RA, Aubin JE, Bai D, Bruneau BG, Grynpas M, Henderson J, Henkelman RM, McKerlie C, Sled JG, Stanford WL, Laird DW, Kidder GM, Adamson SL, Rossant J. A Gja1 missense mutation in a mouse model of Oculodentodigital Dysplasia (ODDD). Development 132 (19): 4375-4386. October 2005.

Tsuchida S, Engelberts D, Roth M, McKerlie C, Post M, Kavanagh BP. Continuous positive airway pressure causes lung injury in a model of sepsis. Am J Physiol Lung Cell Mol Physiol 289 (4): L554-L564. October 2005.

Wood G, Fleming C, Flenniken A, Kassam N, Morikawa L, Porter R, Rossant J, McKerlie C. Two mouse mutations mapped to chromosome 11 with differing morphologies, but similar progressive inflammatory alopecia. Exp Derm 14 (5): 373-379. May 2005.

Kornecki A, Tsuchida S, Ondiveeran HK, Engelberts D, Frndova H, Tanswell AK, Post M, McKerlie C, Belik J, Fox-Robichaud A, Kavanagh BP. Lung development and susceptibility to ventilator-induced lung injury. Am J Resp Crit Care Med 171 (7): 743-752. April 2005.

Gawlik A, Berberovic Z, Wood GA, Flenniken A, Osborne L, Fleming C, Vukobradovic I, McKerlie C, Adamson L, Rossant J, Quaggin SE. Nephrol Dialysis Transplantation 5 (20): 199. June 2005.

Healy A, McKerlie C. Acute Antigen Sensitized Model of Airway Hyper response & Asthma-like Inflammation. The Hospital for Sick Children Research Institute Summer Student Research Day, Toronto, Canada. June 2005.


Serbanescu I, Cortez MA, McKerlie C, OC Snead III. Refractory atypical absence seizures in rat: A two hit model. Epilepsy Res 62 (1): 53-63. November 2004.

McKerlie C, Ayearst R, Fleming C, Liu L, Ottaviani P, Yildiz C. The Canadian Mouse Mutant Repository: A germ cell, embryo, genome, and tissue biorepository. EUMORPHIA 2nd General Assembly, Munich, Germany. October 2004.

Tribl B, Filipp D, Bödeker H, Yu P, Hammerrmüller I, McKerlie C, Keim V, Sibbald WJ. Pseudomonas pneumonia-mediated sepsis induces expression of pancreatitis-associated protein-I in rat pancreas. Pancreas 29 (1): 33-40. July 2004.

Copland IB, Martinez F, Kavanagh BP, Belik J, Engelberts D, McKerlie C, Post M. High tidal volume ventilation causes different inflammatory responses in newborn versus adult lung. Am J Respir Crit Care Med 169 (6): 739-48. March 2004.

Chow RL, Volgyi B, Szilard RK, Ng D, McKerlie C, Bloomfield SA, Birch DG, McInnes RR. Control of late off-center cone bipolar cell differentiation and visual signaling by the homeobox gene Vsx1. PNAS 101 (6): 1754-9. February 2004.

Koehler DR, Sajjan U, McKerlie C, Coates, AL, Tanswell AK, Forstner JF, Hu J. Epithelium-specific gene therapy vector protects cftr knockout mice from acute lung infection. Mol Therapy 9, Supplement 1: S260. June 2004.

Kornecki K, Tsuchida S, Engelberts D, Frndova H, McKerlie C, Belik J, Fox-Robichaud A, Kavanagh BP. Ventilator-associated lung injury - Are infants as susceptible as adults? Am J Resp Crit Care Med 169 (7): A787. April 2004.

Classic Gene Trap Papers

Gossler, A., Joyner, A. L., Rossant, J., and Skarnes, W. C. (1989). Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244, 463-465.

Friedrich, G., and Soriano, P. (1991). Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. Genes & Dev. 5, 1513-1523.

Skarnes, W. C., Auerbach, B. A., and Joyner, A. L. (1992). A gene trap approach in mouse embryonic stem cells: the lacZ reporter is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes & Dev. 6, 903-918.

von Melchner, H., DeGregori, J. V., Rayburn, H., Reddy, S., Friedel, C., and Ruley, H. E. (1992). Selective disruption of genes expressed in totipotent embryonal stem cells. Genes & Dev. 6, 919-927.

Wurst, W., Rossant, J., Prideaux, V., Kownacka, M., Joyner, A. L., Hill, D. P., Guillemot, F., Gasca, S., Cado, D., Auerbach, A., and Ang, S.-L. (1995). A large-scale gene-trap screen for insertional mutations in developmentally regulated genes in mice. Genetics 139, 889-899.

Forrester, L., Nagy, A., Sam, M., Watt, A., Stevenson, L., Bernstein, A., Joyner, A. L., and Wurst, W. (1996). Induction gene trapping in ES cells: Identification of developmentally regulated genes that respond to retinoic acid. Proc. Natl. Acad. Sci. USA 93, 1677-1682.

Expression Trapping

Scherer, C.A., Chen, J., Nachabeh, A., Hopkins, N., Ruley, H.E. (1996). Transcriptional specificity of the pluripotent embryonic stem cell. Cell Growth & Diff. 7,1393-1401.

Stanford, W. L., Caruana, G., Vallis, K. A., Inamdar, M., Hidaka, M., Bautch, V. L., and Bernstein, A. (1998). Expression trapping: Identification of novel genes expressed in hematopoietic and endothelial lineages by gene trapping in ES cells. Blood. 92, 4622-4631.

Hidaka, M., Caruana, G., Stanford, W.L., Sam, M., Correll, P.H., and Bernstein, A. (2000) Hzf and Hhl, two novel megakaryocytic genes identified by hematopoietic-specific gene trapping. Mech. Dev. 90, 3-14.

Gene Trap Vectors

Shigeoka, T., Kawaichi, M., Ishida, Y. (2005). Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells. Nucleic Acids Res. 33(2):e20.

Cobellis, G., Nicolaus, G., Iovino, M., Romito, A., Marra, E., Barbarisi, M., Sardiello, M., Di Giorgio, F.P., Iovino, N., Zollo, M., Ballabio, A., and Cortese, R. (2005). Tagging genes with cassette-exchange sites. Nucleic Acids Res. 33(4):e44.

Skarnes, W.C., Moss, J.E., Hurtley, S.M., and Beddington, R.S. (1995). Capturing genes encoding membrane and secreted proteins important for mouse development. Proc Natl Acad Sci U S A 92, 6592-6596.

Yoshida, M., Yagi, T., Furuta, Y., Takayanagi, K., Kominami, R., Takeda, N., Tokunaga, T., Chiba, J., Ikawa, Y., and Aizawa, S. (1995). A new strategy of gene trapping in ES cells using 3'RACE. Trans. Res. 4, 277-287.

Hicks, G.G., Shi, E.G., Li, X.M., Li, C.H., Pawlak, M., and Ruley, H.E. (1997). Functional genomics in mice by tagged sequence mutagenesis. Nature Genet. 16, 338-344.

Salminen, M., Meyer, B. I., and Gruss, P. (1998). Efficient Poly A trap approach allows the capture of genes specifically active in differentiated embryonic stem cells and in mouse embryos. Dev. Dyn. 212, 326-333.

Zambrowicz, B. P., Freidrich, G. A., Buxton, E. C., Lilleberg, S. L., Person, C., and Sands, A. T. (1998). Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells. Nature 392, 608-611.

Hardouin, N., and Nagy, A. (2000). Gene-trap-based target site for cre-mediated transgenic insertion. Genesis 26, 245-252.

In Vitro Differentiation of ES Cells

Doetschman, T.C., Eistetter, H., Katz, M., Schmidt, W., and Kemler R. (1985). The in vitro development of blastocyst-derived embryonic stem cell lines: Formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morph. 87, 27-45.

Schmitt, R.M., Bruyns, E., and Snodgrass, H.R. (1991). Hematopoietic development of embryonic stem cells in vitro: cytokine and receptor gene expression. Genes & Dev. 5, 728-740.

Wang, R., Clark, R., and Bautch, V.L. (1992). Embryonic stem cell-derived cystic embryoid bodies form vascular channels: an in vitro model of blood vessel development. Development 114, 303-316.

Keller, G., Kennedy, M., Papayannopoulou, T., and Wiles, M.V. (1993). Hematopoietic commitment during embryonic stem cell differentiation in culture. Mol. Cell. Biol. 13, 473-486.

Nakano, T., Kodama, H., and Honjo, T. (1994). Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 265, 1098-1101.

Maltsev, V.A., Wobus, A.M., Rohwedel, J., Bader, M., and Hescheler, J. (1994). Cardiomyocytes differentiated in vitro from embryonic stem cells developmentally express cardiac-specific genes and ionic currents. Circ Res, 75,233-44.

Bautch, V.L., Stanford, W.L., Rapoport, R., Russell, S., Byrum, R.S., and Futch, T.A. (1996). Blood island formation in attached cultures of murine embryonic stem cells. Dev. Dyn. 205, 1-12.

Choi, K., Kennedy, M., Kazarov, A., Papadimitriou, J.C., and Keller, G. (1998). A common precursor for hematopoietic and endothelial cells. Development 125, 725-732.

R1 ES Cells

Nagy, A., Rossant, J., Nagy, R., Abramow-Newerly, W., and Roder, J.C. (1993). Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc Natl Acad Sci U S A. 90, 8424-8428.

Learning & Memory

Gingrich, J. and RODER, J.C. (1998). Inducible gene expression in the nervous system of transgenic mice". Ann. Rev. Neuroscience 21:377-405.

Jia ZP, Agopyan N, Miu P, Xiong Z, Henderson J, Gerlai R, Taverna F, Velumian A, MacDonald J, Carlen P, Abramow-Newerly W. and RODER, J.C. (1996). Enhanced LTP in mice deficient in the AMPA receptor, GluR2. (1996). Neuron 17:945-956. Li, C., Tropak, M., Gerlai, R., Clapoff, S., Abramow-Newerley, W., Trapp, B., Peterson, A., and RODER, J.C. (1994) Myelination in the absence of MAG. Nature 369:747-750.

Lu, YM, Mansuy, I, Kandel, E and RODER, JC. (2000). Calcineurin-mediated LTD of GABAergic Inhibition Underlies the Increased Excitability of CA1 Neurons Associated with LTP. Neuron 26:197-205.

Lu, Y.M., RODER, J.C., Davidow, J. and Salter, M.W. (1998). Src activation mediates induction of long-term potentiation in hippocampus. Science 279:1363-1367.

Nagy, A., Rossant, J., Nagy, R., Abramow-Newerly, W. and RODER, J.C. (1993) Derivation of completely cell culture-derived mice from early passage embryonic stem cells. PNAS 90:8424-8428.

Mount Sinai Hospital