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Centre for Modeling Human Disease
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ENU Pathology Assays

Gross pathology

Detailed anatomic pathology including macroimaging and organ morphometrics.

G1 animals - a routine post-mortem examination that includes a complete physical exam of live and euthanized animals, collection of appropriate samples for additional analysis (e.g. blood for CBC), a systematic necropsy, and recovery of any gross lesions for histologic evaluation 

G2 mutants - a complete post-mortem examination that includes a routine necropsy plus gross CNS evaluation, recovery of any gross lesions plus phenotyped-related tissue, macroimaging, and systematic recovery of 40 tissues for histologic evaluation

Histopathology

Specialized histopathology of all organ systems including microimaging and tissue morphometrics.

G1 animals - systematic processing and examination of any gross lesions recorded at post-mortem

G2 mutants - systematic processing and examination of 40 tissues (including CNS) with bright-field microscopy and hematoxylin & eosin stained tissue sections. Additional techniques may include special tissue staining, immunohistochemistry (CD antigens, proliferation, apoptosis), morphometry, or ultrastructural examination

Archiving

Tissue and sperm are collected and archived in our Mouse Mutant Repository

Frozen tissue - fresh tissue (liver) is systematically harvested at necropsy from G0, G1 and G2 animals and is snap frozen into our frozen-tissue archive in liquid nitrogen 

Sperm - spermatozoa from all G0, G1 and some G2 males are cryopreserved and archived in liquid nitrogen for future requirements 

Ova - ovaries from any G1 or G2 females with an in-life phenotype of interest are cryopreserved and archived in liquid nitrogen for future requirements 

Fixed tissue, paraffin-embedded tissue, and glass slide sections - fixed and processed tissue from all animals examined are archived and available for future reference

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