| Primer Sequences for GeneTrap Vectors (CMHD) | |||||||||||||
| Primer Name and It's Use | |||||||||||||
| Vector Name | 3'RACE RT | 3'RACE (1st) | 3'RACE (2nd) | 3'RACE Seq | 5'RACE RT | 5' RACE (1st) | 5' RACE (2nd) | 5'RACE Seq | Extra or Comments | ||||
| pBgal | GTlacZ1+SM2 | 5CD2 | 5'RACE1 | 5CD2, 5'RACE1 | 5'RACE for both + & - | ||||||||
| 10x UPM | Nest1 | ||||||||||||
| pGT1 | 3CDs | SD5-P1 | SD5-P3 | SD5-P3, 3'RACE1 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| pGT6 / 7 / 8 | 3CDs | SD5-P1 | SD5-P3 | SD5-P3 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| pGTlox4 | 3CDs | pGTlox4-1 | pGTlox4-2 | pGLox4-P4 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| pMS1 | 3CDs | SD5-P3 | 3'RACE1 | 3S1, SD5-P3, 3'RACE1 | GTlacZ1[1] | 5CD2 | 5'RACE1 | 5CD2, 5'RACE1 | |||||
| 10x UPM | Nest1 | 10x UPM | Nest1 | ||||||||||
| pMS2 / 3 | 3CDs | SD5-P3 | 3'RACE1 | 3S1 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| pNH94 | GTlacZ1[2] | GTlacZ1[3] | 5CD2 | 5'RACE1 | 5CD2, 5'RACE1 | only for LacZ+ 5RACE | |||||||
| 10x UPM | Nest1 | ||||||||||||
| pNH120 | 3CDs | SD5-P3 | 3'RACE1 | 3S1, SD5-P3, 3'RACE1 | GTlacZ1[4] | 5CD2 | 5'RACE1 | 5CD2, 5'RACE1 | same as pMS1 | ||||
| 10x UPM | Nest1 | 10x UPM | Nest1 | ||||||||||
| pNMDi3 / 4 | 3CDs | SD5-P1 | SD5-P3 | SD5-P3 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| pGepNMDi3 | 3CDs | SD5-P1 | SD5-P3 | SD5-P3 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| pPT1 | 5CDs or GTlacZ1 | 5CD2 | 5'RACE1 | 5CD2, 5'RACE1 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| SD5 | 3CDs | SD5-P1 | SD5-P3 | SD5-P3 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| pGepSD5 | 3CDs | SD5-P1 | SD5-P3 | SD5-P3 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| pUPA | 3CDs | pUPAtrap6 | pUPAtrap3 | pUPAtrap7 | |||||||||
| 10x UPM | Nest1 | ||||||||||||
| List of Primers used for Genetrap (CMHD) | |||||||||||||
| Primer Name | Primer Sequence 5'--> 3') | ||||||||||||
| 3CDs | AAG CAG TGG TAA CAA CGC AGA GTA CTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TVN | ||||||||||||
| GTlacZ2 | GAC TCT GGC GCC GCT GCT CTG TCA G | ||||||||||||
| SM2 | AAG CAG TGG TAA CAA CGC AGA GTA CGC GGG | ||||||||||||
| 5CDs | TTT TTT TTT TTT TTT TTT TTT TTT TVN | ||||||||||||
| 10x UPM | 0.4 μM UPL + 2 μM UPS (2.5μl in 25μl reaction) | ||||||||||||
| UPL | CTA ATA CGA CTC ACT ATA GGG CAA GCA GTG GTA ACA ACG CAG AGT | ||||||||||||
| UPS | CTA ATA CGA CTC ACT ATA GGG C | ||||||||||||
| Nest1 | AAG CAG TGG TAA CAA CGC AGA GT | ||||||||||||
| SD5-P1 | GCT TGC CGA ATA TCA TGG TGG AAA ATG G | ||||||||||||
| SD5-P3 (3'RACE7, 3pMS1-1) | CGC ATC GCC TTC TAT CGC CTT CTT GAC G | ||||||||||||
| 3'RACE1 (3pMS1-2) | CAA GCG ACG CCC AAC CTG CCA TCA CGA G | ||||||||||||
| 3S1 (sequence1) | CGG GGA TCT CAT GCT GGA | ||||||||||||
| pUPAtrap6 | TAT AGC ATA CAT TAT ACG AAG TTA TCC GCT CGA C | ||||||||||||
| pUPAtrap3 | GCC ATG CAC CAT GGT TCG AAA TTC CAG | ||||||||||||
| pUPAtrap7 | ATT CCA GAC AAG TTT GTT GTT GGA TAT GCC | ||||||||||||
| 5CD2 (5PT-1, 5PT-P1) | GAC TCT GGC GCC GCT GCT CTG TCA GGT | ||||||||||||
| 5'RACE1 (5PT-2, 5PT-P2 ) | ACC TGT TGG TCT GAA ACT CAG CCT TGA G | ||||||||||||
| pGLox4-P1 (pGLox1) | CCG CAT CTT CAC TGG TGT CAA TGT ATA | ||||||||||||
| pGLox4-P2 (pGLox2) | TAT ATC ATT TTA CTG GGG GAC CTT GTG C | ||||||||||||
| pGLox4-P4 (pGLox4) | AAG CCA TAG TGA AGG ACA GTG ATG GA | ||||||||||||
| Nested2 | CCT GTT GGT CTG AAA CTC AGC CT | ||||||||||||
| bsdL | GTTATGTGTGGGAGGGCTAAGCAAT | ||||||||||||
| NeoL | GCTATCAGGACATAGCGTTGGCTAC | ||||||||||||